Enzyme purification is a key biological separation process, which obtains high purity and high activity enzymes by purifying the target enzymes from complex mixtures. In the enzyme purification process, it is crucial to choose the right separation technology and equipment, and peristaltic pumps are a highly respected equipment choice.

Peristaltic pump has become an indispensable tool in enzyme purification process because of its unique working principle and excellent purification effect. It promotes fluid transmission in the pipeline by means of mechanical peristalsis, avoiding the problem of the introduction of gas in the transport process of the traditional pump, and ensuring the purity of the enzyme. The advantage of peristaltic pumps compared to other conventional pumps is that they can provide a more accurate and stable flow rate, ensuring precise control of the enzyme purification process.

In the enzyme purification process, the work flow of the peristaltic pump is relatively simple, but its performance is very good. First, the enzyme mixture is fed through a peristaltic pump to the separation column. Next, in the separation column, the enzymes interact with the affinity matrix fixed in the column, and there are specific binding between different enzymes and the affinity matrix. By controlling the fluid velocity and retention time, the non-target substances can be eluded and the target enzymes can bind specifically to the affinity matrix. Finally, high purity target enzyme was obtained by elution with appropriate elution buffer.

The advantages of peristaltic pumps in the enzyme purification process are not only reflected in the purity effect, but also in the ease of operation and maintenance. Peristaltic pump because of the valveless structure, to avoid the liquid flow of leakage and cross contamination of the hidden dangers, maintenance only need to replace the peristaltic pump head, more simple and convenient. In addition, the peristaltic pump uses a digital control system, which can realize automatic control and remote monitoring, improving the reliability and stability of the enzyme purification process.

To summarize, enzyme purification peristaltic pumps have unique advantages in the biological separation process. It not only ensures the efficiency and accuracy of enzyme purification, but also has the convenience of operation and maintenance. Both researchers and manufacturers should realize the importance of peristaltic pumps in enzyme purification and make full use of them.